منابع مشابه
Enzymatic assay of D-mannose in serum.
We describe a new and improved enzymatic assay for determining the concentration of D-mannose in sera. Serum D-glucose is selectively converted to glucose-6 phosphate with the highly specific thermostable glucokinase (EC 2.7.1.2) from Bacillus stearothermophilus. The anionic reaction products and excess substrates are removed by a rapid and simple anion-exchange chromatography step in microcent...
متن کاملEnzymatic determination of D-mannose in serum.
A new and simple enzymatic assay for measuring D-mannose in serum is described. Endogenous glucose is eliminated from serum by use of glucose oxidase (EC 1.1.3.4) and catalase (EC 1.11.1.6). D-Mannose concentration is calculated from the increase in NADH formation after mannosephosphate isomerase (EC 5.3.1.8) is added. This increase is a result of coupling the following series of enzymes: hexok...
متن کاملEnzymatic determination of unbound D-mannose in serum.
Mannose is an aldohexose component of a number of glycoproteins in cellular membranes and blood plasma. Free (unbound) mannose is a normal blood plasma constituent and its concentration is elevated in diabetes mellitus and chronic glomerulonephritis. We devised an enzymatic method for the determination of free mannose in which mannose is converted to glucose-6-phosphate and measured spectrophot...
متن کاملEnzymatic assay of calcium in serum with phospholipase D.
(10) of 2.0 g/L and Holt et al. (12) of Ͻ3.0 g/L. HPLC-MS has no detectable interference with respect to endogenous compounds. The quantification limits based on acceptable imprecision, recovery, and ability to discriminate from potential interferences within the calibration range of the MEIA are 3.0 g/L (the lower limit of quantification) to 22.0 g/L. We have demonstrated that a higher lim...
متن کاملNew enzymatic assay of iron in serum.
A new enzymatic method for assaying iron in serum samples, suitable for automated analyzers, is reported. Three reagent mixtures are used: dilution buffer (pH 3.0; ascorbate), reagent 1 (pH 6.7; apoaconitase), and reagent 2 (pH 7.7; citrate, magnesium, and isocitrate dehydrogenase). Sera are diluted with dilution buffer. Fe3+ is liberated from transferrin in sera under acidic conditions, and th...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1997
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/43.3.533